Association of P53 expression with clinicopathological gradings of oral squamous cell carcinoma patients in Hyderabad Sindh.

Abstract

Objective: To evaluate the frequency and association of p53 gene with increase histopathology categorizing of “oral squamous cell carcinoma” patients. Study Design: Comparison Cross-sectional Research. Setting: Isra Dental College Hyderabad. Period: 1 November 2021 to 31 October 2022. Material & Methods: Sixty patients of identified oral squamous cell carcinomas were chosen by consecutive sampling from patients reporting at Oral surgery department Isra dental college, Poly-L-lysine solutions was used over all of the formalin-fixed, paraffin embedded segments, and was prepared for immunostaining. Primary antibody and counter stain hematoxylin was used and dehydrated through graded alcohol dilutions. Sections were mount by Dpx and results interpreted. Results: Sixty instances of oral squamous cells carcinoma divided into histological grading well differentiated OSCC, moderately differentiated OSCC, and poor differentiated OSCC based on their histological characteristics. Fifty-two (87%) of the participants in this study were male, while just eight (13%) were females. Association of p53 were found insignificant with age. Gender and other clinical factors of OSCC, the prevalence of p53 gene countenance was found increase in oral squamous cell carcinoma. But there was no any significant association found with the increase histological grading of OSCC. Conclusion: Prevalence of P53 gene expression found increase in OSCC. While P53 expression was not significantly found with increase histological grading of oral squamous cell carcinoma.