Occurrence of anti D alloantibodies among weak D and D negative patients.

Abstract

Objective: To estimate the frequency of anti-D alloantibodies in patients with weak or negative D. Study Design: Cross-sectional study. Setting: Under CIP/IRB/1111 in the Blood Bank of CHUGHTAI Institute of Pathology. Period: May 2022 to October 2022. Material & Methods: Patients who booked their blood group testing at this laboratory were tested for their ABO RH blood type. The EDTA and yellow top serum samples were taken from patients. Blood group was identified using tube method. Patients with Rh-D negative blood type and weak D were included in this study. Those patients were screened for alloantibodies by screening cell (Bio-Rad), and those patients whose screening came out positive were tested for antibody identification using an extended 11cell panel (Bio-Rad). The Statistics calculator was used to determine the sample size. Software SPSS version 23 was used to conduct all statistical analyses. Results: 14103 blood groups were tested. The age of patients on average was 51±22 years. The males and females were 44% and 56%, respectively. Out of n=14103, 9.5% came out Rh-negative blood type. The blood group distribution was as follows, B negative 46% O negative 23% A negative 20% AB negative 11%, and weak D 0.02% only. Total 7.1% participants with negative RH-D blood type had Rh alloantibodies detected. Patients with weak D had no Rh alloantibody. Conclusion: Blood group O RH-D negative was the most prevalent with positive anti D-alloantibodies. Prevalence of positive antibody was 7.1%.