Topical probiotics and steroids can accelerate the process of angiogenesis in wound repair: A comparative study in rats.

Abstract

Objective: To asses if Topical probiotics and steroids can accelerate the process of angiogenesis in wound repair. Study Design: Longitudinal Experimental study. Setting: Al-Tibri Medical College. Period: Jan 2020 to Dec 2020. Material & Methods: Total 72 male albino rats were acquired from the animal house of the institution, and 18 as a requirement of per day of sampling included 6 animals/gp. An equal size incision of (2x2cm2) was made in all the animals. Group A was given Topical Normal Saline (Control Group), Group B was given the Probiotic (Lactobacillus Acidophilus), and Group C was given Topical Steroid (Dexamethasone). Samples from the wound created surgically was obtained on the 3^rd, 7^th, 14^th, and 21^st Day and slides were created to observe the number of blood vessels formed and angiogenesis under a light microscope. Data was analyzed using SPSS version 21.0. To compare the mean between the groups, the ANOVA was applied along with post hoc Tukey's test. The level of significance was considered P≤0.05. Results: The mean number of blood vessels formed in Group A (Control Group) was 2.75, 4, 5.25, and 5.50, in Group B (Probiotics Group) was 10, 13.50, 2.25, and 1, and in Group C (Steroid Group) was 3.75, 5.50, 3, and 2.25 on the 3^rd, 7^th, 14^th, and 21^st Day respectively. Significant difference was seen in the mean number of blood vessels formed when the Probiotic group was compared to Group A and C. Conclusion: Topical Probiotic accelerates the process of angiogenesis in wound repair due to their anti-inflammatory response.