Resurgence of sperm motility: An effect of antioxidant oils in phenytoin induced toxicity of rat testis.

Abstract

Objective: To evaluate the restoration of sperm motility with the antioxidant oils (Virgin coconut oil and Corn oil) therapy in Phenytoin induced testicular toxicity of rats. Study Design: Experimental Study. Setting: Al-Tibri Medical College and Hospital. Period: November 2019 to March 2020. Material & Methods: Forty-eight male albino rats weighing between 150-250gms were taken from the animal house of Al-Tibri medical college and placed in 4 groups, each with 12 rats. Group A was the control group receiving saline, Group B received Phenytoin only, Group C received Phenytoin and Virgin coconut oil, and Group D received Phenytoin and Corn oil. The rats were euthanized on the 4^th, 5^th, and 6^th week of the study. Their epididymis was cut, and the epididymal fluid was placed under the microscope to determine sperm motility. Data was analyzed using SPSS Version 20.0 with one-way ANOVA followed with Post-Hoc Tukey’s test to compare statistical significance. Results: The significant difference was seen in mean % sperm motility when comparing Group B vs. A (≤0.001) and Group B vs. C (≤0.001) during the 4^th, 5^th, and 6^th. Conclusion: Virgin Coconut Oil and Corn Oil both restored sperm motility. A higher percentage was observed in Virgin Coconut Oil received rats, which proved effective in resurging sperm motility.