HEPATITIS C VIRUS;

SENSITIVITY OF ANTI HEPATITIS C VIRUS ANTIBODIES THRU ELISA METHOD IN COMPARISON TO HEPATITIS C VIRUS RIBONUCLEIC ACID BY PCR METHOD.

Abstract

Objectives: The plan of this current research was in the direction for towards the
assessment of the existing ELISA (Enzyme Linked Immunosorbant Assay) method through
antibodies testing for identification of hepatitis C virus disease by comparing their outcome with
the Real Time polymerase chain reaction analysis. Setting: Peoples Medical College Hospital
Nawabshah. Period: December 2015 to December 2016. Methods: In this current research 100
blood samples were analyzed due to the presence of anti-HCV antibodies by 3rd-generation
enzyme-linked immunosorbent assay testing. All the specimens were 100% positive. Polymerase
chain reaction test was performed according to the laboratory directions in anti- hepatitis C virus
antibodies positive patients to validate the diagnosis of hepatitis C virus infectivity. Results: This
research shows that, the entire results were positive by Enzyme Linked Immunosorbant Assay
testing. As compared with polymerase chain reaction the of Enzyme Linked Immunosorbant
Assay in this research the screening test for anti hepatitis C virus - antibodies is about 2%
false positive. Out of the 100 samples 98 cases are positive by Real Time polymerase chain
reaction analysis while only 02 cases report are negative (2%). Conclusion: The proportion of
hepatitis C virus infectivity was 100% by 3rd-generation enzyme-linked immunosorbent assay
testing, 98% by Real Time polymerase chain reaction analysis. As in our research the hepatitis
C virus –Ribonucleic acid is present in 98% cases who are the Anti- hepatitis C virus antibodies
positive patients, it can be suggested that Anti-HCV antibodies detection by third generation
ELISA technique in routine procedure is sufficient to determine HCV infection.