Expression of KRAS in tissue samples of colorectal carcinoma and its correlation with various histo-pathological parameters.

Abstract

Objective: To assess the expression of KRAS in tissue samples of colorectal carcinoma and to correlate it with histopathological parameters. Study Design: Cross Sectional study. Setting: Department of Pathology, PNS Shifa Hospital Karachi. Period: March 2016 to February 2019. Material & Methods: A total of 51 cases of CRC were analyzed for immunohistochemical staining using KRAS antibody on representative tissue blocks. Clinical and pathological records were retrieved for collection of data. The results of immunohistochemical analysis were correlated with the recorded clinico-pathological parameters. Results: In this study 51 cases of CRC were analyzed for immunoexpression of KRAS. The age of the patients ranged from 14 to 85 years with the mean age of 60.96 years. Among the 51 cases, 37(72.5%) cases were males and 14(27.4%) were females. 37(72.5%) were localized to left side colon and 14(27.4%) were found in the right colon. For KRAS immunostaining, 41(80.3%) out of 51 cases showed overexpression. Significant association was seen between KRAS overexpression and histological variants i.e. glandular carcinomas. Conclusion: In the present study over expression of KRAS was observed in advanced tumors. Majority of these cases were adenocarcinomas with few showed mucinous histology. The present study signifies that established KRAS expression is usually seen in rapidly dividing cells with association of advanced tumors.