HER-2/neu OVER EXPRESSION
IMMUNOHISTOCHEMICAL DETERMINATION IN INVASIVE LOBULAR CARCINOMA OF BREAST
DOI:
https://doi.org/10.29309/TPMJ/2002.9.03.5410Keywords:
HER-2/neu, immunohistochemistry, invasive lobular carcinomaAbstract
O BJECTIVE: To determine the frequency of HER-2/neu over expression in invasive lobular carcinoma (ILC)
of breast & correlate it with various standard prognostic parameters. DESIGN: This study was performed on formalin fixed paraffin embedded blocks of 18 diagnosed cases of invasive carcinoma of breast with classic lobular pattern. PLACE: Department of Pathology, Basic Medical Science Institute, Jinnah Postgraduate Medical Center, Karachi, Pakistan. METHODS: The paraffin block were retrieved and 5:m thick sections were cut and stained with H&E for the review of diagnosis and grading. The immunohistochemical staining was done on 4 :m thick sections by using polyclonal rabbit anti HER-2 ZYMED USA, and ZYMED 2nd generation LAB-SA immunodetection system, to see the HER-2/neu over-expression. RESULTS: Sections containing > 50% of tumor cells exhibiting intense circumferential cell membrane staining were scored as positive. The HER-2/neu over expression was seen in 22% of cases. CONCLUSION: The results in this study are statistically insignificant but positive cases can get benefit from Herceptin therapy.
