HYPO-OSMATIC SWELLING TEST
DOI:
https://doi.org/10.29309/TPMJ/2002.9.02.5401Keywords:
Non motile sperms, HOS Test, ICSIAbstract
H ypo-Osmotic Swelling (HOS) test is considered as a reliable method to find out live sperms. OBJECTIVE:
To evaluate the effect of HOS test for selection of alive sperms. DESIGN: Prospective randomized study.
PERIOD: June 2000-March 2002. SETTING: Infertility Advisory Centre Lahore. METHODS AND
RESULTS: Twenty four ICSI cycles were included in the study. In all the cases the sperms were non motile they
were divided in two groups. In group A (12 cases) sperms were used which were tested by HOS and labeled as
alive sperms. In group B (12 cases) in which sperms were randomly selected (without HOS Test) and used in ICSI
Fertilization, embryo quality and clinical pregnancy rate were compared in two groups. CONCLUSIONS: HOS
test is an effective and safe method to be used for selecting alive sperms in cases of necrospermia when they are
used in Intracytoplasmic sperm injection (ICSI)to achieve a pregnancy.