PROTECTIVE ROLE OF ANTI-OXIDANT

AGAINST HEPATOTOXICITY BY CIPROFLOXACIN IN WISTAR ALBINO RATS

Abstract

Objectives: To evaluate the preventive role of zinc chloride on hepatotoxicity of Ciprofloxacin administration in Wistar albino rat pups.
Design of Study: Prospective and Comparative study. Setting: The study was carried out in the department of Anatomy, Basic Medical
Sciences Institute, Jinnah Postgraduate Medical Centre, Karachi. Period: From 2001 to 2002. Material & Method: Ciprofloxacin and zinc
chloride were administered to newly born albino rat pups separately and simultaneously at a dose of 20 mg/kg body weight and 120 ug/100
gm body weight respectively intraperitoneally twice daily from day-1 to day-14 after birth. Pups weighed on postnatal day-1 and postnatal
day-14, on day-15 after birth these animals were killed by deep ether anaesthesia, their liver removed, weighed, and fixed in 10% buffered
formalin, embedded in paraplast, 3 um thick sections were cut on rotary microtome and stained with H&E. The histomorphological features
of livers were compared with those of control animals and analyzed statistically. Results: The study revealed that Ciprofloxacin
administration in new born albino rat pups produced necrotic changes in liver and simultaneously zinc chloride partially prevented
hepatotoxicity. Conclusion: These results strongly suggest that Ciprofloxacin causes severe liver damage in pups. However Ciprofloxacin
induced hepatotoxicity could be partially prevented by simultaneous administration of zinc chloride in Wistar albino rat pups.