Ameliorative effect of Alpha-tocopherol on E-cigarette liquid induced histomorphological changes in adrenal cortex of male Albino rats.

Abstract

Objective: To study the ameliorative effect of Alpha-Tocopherol (Vitamin E) against E-cigarette induced histomorphalogical changes in adrenal cortex of male Albino rats. Study Design: Randomized Animal Control Trial. Setting: Department of Anatomy, Islamic International Medical College Rawalpindi in collaboration with National Institute of Health (NIH), Islamabad. Period: September 2018 to September 2019. Material & Methods: 30 adult male albino rats were divided in three groups having 10 rats each. Normal diet and distilled water was given to control group (Group I) including 10 rats, for 4 weeks. The Rats of Group II were injected E-cigarette liquid 0.5mg per kg body weight per day diluted in 500ul normal saline intraperitoneally for 4 weeks. The experimental rats in Group III, in addition to E-cigarette liquid also received Vitamin E, 100mg per kg body weight per day mixed in daily diet. Dissection of the animals was done after completion of 4 weeks and adrenal gland was removed for histological analysis. Data was recorded and analyzed in SPSS™ version 21. Results: Alpha-Tocopherol restored the quantitative changes induced by E-Cigarette liquid in adrenal cortex in Group III, which was increased in the rats of Group II who were injected E-Cigarette liquid. Among microscopic parameters Orientation of cells, sinusoidal dilatation was significantly reduced in all adrenal cortex zones by Alpha-Tocopherol in Group III animals. Conclusion: The current study demonstrated that Alpha-Tocopherol had an ameliorative effect on histological changes caused by E-cigarette liquid on adrenal cortex.