ARSENIC INDUCED MICROSCOPIC CHANGES IN RAT TESTIS
,,
DOI:
https://doi.org/10.29309/TPMJ/2008.15.02.2760Keywords:
PAS-Sulfurous, Haematoxylin Stain,, Arsenic Toxicity,, Seminiferous Tubules, Spermatogenic Cells, Leydig cells and atrophic changes in testis.Abstract
The present study was designed to observe the changes in the testis of rats due to arsenic in higher
doses. Distilled water and sodium arsenite were administrated intra-peritonealy to control and experimental groups
respectively. Animals were sacrificed, their testis were weighed and cut into small pieces. After observing the plucking
and stringing phenomenon of the seminiferous tubules the pieces of tests were embedded in paraffin and then 5µm
thick section were made. These sections were stained with PAS-sulfurous acid haematoxylin and examined
microscopically for qualitative assessment of germinal epithelium. Results: In the rats of experimental group mean
weight and average tissue ratio of the paired testes was 1.140gm and 0.0037 respectively, which was significantly less
than the control. There was decrease in diameter of seminiferous tubules, thickening of the basement membrane, early
arrest of spermatogenesis, damaged leydig cells, prominent sertoli cells and collapsed blood vessels, showing
generalized atrophy of the testes in experimental group. Conclusion: In arsenic toxicity there are atrophic changes
in testis due to degenerative changes in spermatogenic and leydig cells
