METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA);

PREVALENCE AND SUSCEPTIBILITY PATTERN OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) ISOLATED FROM PUS IN TERTIARY CARE OF DISTRICT HOSPITAL OF RAHIM YAR KHAN.

Abstract

Background:Methicillin resistant Staphylococcus aureus (MRSA) is one of the notorious bugs to cause nosocomial human infections worldwide. However its prevalence and susceptibility pattern quite varies in hospitals among different countries or within same country.Objectives: To study the prevalence and antibiotic susceptibility pattern of MRSA isolated from pus sample.Study Design: Cross sectional study.Period:6 months. Setting:Tertiary care hospital of district Rahim Yar Khan -Punjab, Pakistan at its microbiology laboratory.Materials and Methods:A total 100 clinical specimen of pus and wounds were collected from various indoor and outdoor department of this institute. .The Staphylococcus aureus was identified on the basis of colony morphology, Gram’s stain, and biochemical tests.Antibiotic susceptibility pattern was done according to CLSI guidelines.Results:Out of 100 clinical sample of pus and wounds, 21 coagulase positive staph. aureus were isolated, 14 (66.7%) strains were found to be methicillin resistant and 07(33.3%) were found to be methicillin sensitive. Higher percentage of MRSA was isolated from surgical unit (66.7%), indicating its nosocomial source of spread. Maximum resistance to MRSA isolate were developed to Amoxicillin/ clavulinic acid (80%) followed by ofloxacin(71%), cefoxitin (66.7%), tetracycline (58%), Ciprofloxacin (48%), Erthromycin (46%) andAmikacin (40%).However, all strainswere sensitive to vancomycin.Conclusion: There is high frequency of resistance among coagulase positive Methicillin resistant staph.aureus.Regular surveillance of hospital-associated infection and frequent monitoring of antibiotic sensitivity pattern is required to reduce MRSA prevalence.