DIVERSITY OF HEPATITIS C VIRUS

ANALYSIS OF GLYCOPROTEINS E1 AND E2

Abstract

Objective: (1)To understand the diversity in HCV glycoprotein E1 and E2
sequences at different stages of infection. (2) Method widely used in evolutionary studies of HIV,
as a new gold standard in HCV research.Setting: Department of Biochemistry Sir Syed Institute
st of Medical Sciences Karachi. Period. 1 Jan 2012 to 31 Dec 2012. Design: Experimental
analysis. Methods: The samples derived from a xenomouse model of transmission and several
samples from naturally occurring transmission as well as sequences from acute stage of
infection. we utilized single genome amplification (SGA) technique to recover full- length E1
sequences. SGA of full-length E1 glycoprotein sequences, followed by direct sequencing
minimizes in vitro generated artifacts and experimental biases associated with the standard bulk
amplification and cloning approach, giving an accurate representation of investigated intrahost
population. Analysis and results: Comparative analyses of sequences derived from three
different settings were performed, in conjunction with a range of phylogenetic tests. As full-length
E1 sequences were utilised. Conclusions: The role of glycoprotein E1 and E2 during the
infection of HCV was Known. The new gold standard in HCV research prove to be of great
importance.