MIDSTREAM URINE ISOLATES

SENSITIVITY PATTERN OF VARIOUS ANTIBIOTICS IN PEDIATRIC PATIENTS

Abstract

Objectives: The objectives of the study was to determine different
microorganisms responsible for causing urinary tract infections UTI in children and to evaluate
sensitivity and resistance pattern of different antibiotics used in UTIs. Setting: Study was
conducted in Children Hospital Complex (CHCM), Multan, Pakistan. Methods: Total 125 children
having UTI were taken to determine the antibiotic sensitivity and resistance pattern against
microorganisms causing urinary tract infections in children. The urine samples were collected in
urine bottles and smears were made within 2 hours of sample collection. Urine sample was
inoculated on agar media and then incubated for 24 hours. A smear was prepared on a slide form
culture of microorganisms and gram testing was conducted. The microorganisms were then
characterized by use of API (Analytical Profile Index) MICROBACT TM 24E of Oxoid England. The
antimicrobial susceptibility testing of various antibiotics was performed by disc diffusion method.
Results and Conclusions: The percentage of Gram +ve bacteria causing urinary tract infections
in children was 28%, Gram –ve 62.4% and Yeast 9.6%. The most prevailing species are S. aureus,
S. epidermidis and E.coli. Results showed that female children are more prone to UTI than male
children. Norfloxacin is effective in S. Aureus UTI while cefaclor is effective in S.epidermidis UTI.
Amikacin, Norfloxacin and Cefuroxime are effective in UTI caused by E.coli.