EFFECT OF CAPTOPRIL

GLYCATION INHIBITION IN NORMAL AND DIABETIC PLASMA BY CAPTOPRIL

Abstract

Objectives: (1) To investigate the inhibitory effect of Captopril on level of glycation (in vivo). (2) To study glycation inhibition in
vivo. Study design: Case study. Period: Sep. 2006 to March. 2008. One year seven months. Setting: Department of Biochemistry University
of Agriculture, Faisalabad. Methods: Different parameters like fluorescence, total proteins, TBA (thiobarbituric acid) method, periodate
borohydride assay were used to check the effect of inhibitor on glycation. Thirty two combinations were made and all these combinations were
placed at 37̊C, at same time for five weeks. 3mL of blood sample was drawn after 1st, 3rd and 5th week of incubation to perform the experiments
for glycation and glycation inhibition. Along with the same temperature (37̊C), different combinations of glucose and inhibitor were used.
Results: Effective concentration of inhibitor helped to decrease the level of glycation. All concentrations of glucose (G , G and G ) showed 1 2 3
glycation with protein. The inhibitor Captopril (all concentrations) showed variations in inhibition of glycation at one temperature (37̊C) with
different parameters (Fluorescence, TBA and Periodate) but the most effective concentration of inhibitors at each condition is I (1mM) but I (10 3 1
mM) and I (5 mM) were also equally effective after I . Periodate borohydride Assay is more effective for glycation determination than 2 3
thiobarbituric acid assay. Conclusions: Captopril can be used as glycation inhibitor in future. As it enhances the activity of transketolase, it can
produce 3DG compound which can block the AGEs. However, more experimentations should be done on animal or on large scale before its
application in diabetic patients.