DIABETIC ALBINO RATS

BLOOD GLUCOSE, SUPEROXIDE DISMUTASE AND GLUTATHIONE PEROXIDASE IN CINNAMON CASSIA TREATED

Abstract

Objectives: The present study evaluated the blood glucose, superoxide
dismutase (SOD) and glutathione peroxidase (GPX) in Cinnamon cassia bark extract (CCBE)
treated alloxan-induced diabetic albino rats. Study Design: Experimental study. Setting: Animal
house of Al-Tibri Medical College, Karachi was selected for conducting the present study.
Period: January 2014 to December 2014. Methodology: A sample of twenty rats was randomly
divided into 4 groups. Rats were grouped as A1 (n=5) – Negative control, A2 (n=5)- Positive
controls (alloxan treated rats), and experimental groups B1 (n=5) and B2 (n=5) included
diabetic rats which received 0.3 grams and 0.6 grams of CCBE respectively. Intraperitoneal
alloxan (120 mg /kg in 0.5 ml dissolved in acetate buffer) was used for induction of diabetes.
C. cassia bark extract was prepared under standard protocol. Blood glucose (glucose oxidase
method), SOD and GPX (Elisa kit) were detected from blood samples after 30 days. Data was
analyzed on SPSS. Data was analyzed by one- way ANOVA and post-Hoc was used for group
comparisons. Data was analyzed at 95% CI (p< 0.05). Result: Blood glucose, SOD and GPX
showed statistically significant differences (p<0.036). CCBE treated rats showed low glucose
levels and elevated SOD and GPX enzyme activity (p<0.05).