HEPATOCELLULAR CARCINOMA

BIOMARKERS: A MOLECULAR APPROACH FOR EARLY DETECTION

Abstract

Objectives: To determine the expression of miR-92-1 in HCC patients and correlate
them with clinicopathological data. Study Design: A Cross sectional study. Place of Study:
Samples were collected from Jinnah Postgraduate Medical Center Karachi, Civil Hospital
Karachi and Asian Institute of Medical Sciences Hyderabad. Duration of Study: January
2014 to December 2014. Materials and Methods: 150 patients of hepatocellular carcinoma
were divided into two groups on the basis of etiologic agent. HCC patients with chronic viral
hepatitis B were labeled as group-I, whereas those with chronic viral hepatitis C in group-II,
compared with 75 healthy control individuals in group-III. Results: The results showed that
miR-92-1 expression was decreased in patients with HCC when compared with controls. Down
regulation was seen more pronounced in HCC cases with chronic viral hepatitis C. A significant
correlation was found between the expression of miR-92-1 and AFP level 20-200 ng/ml and
Child Pugh score B. However no correlation was found between the expression of miR-92-1
and age, gender, fibro scan, tumor distribution, tumor size, tumor metastasis and BCLC stage.
Conclusion: miR-92-1 is down regulated in HCC patients with significant correlation with serum
AFP level between 20-200 ng/ml.