ASSESSMENT OF POTENTIAL RISK FACTOR AND ANTIOXIDATIVE STATUS IN VITAMIN-D DEFICIENT FEMALES.
Keywords:Osteoporosis,, vitamin D,, CAT,, GSH,, SOD,, GPx and GR,, MDA.
Background: Deficiency of vitamin D is characterized by the low bone mass
which leads to the bone fragility and high risk of fractures. Bone fractures causes the formation
of free radicals, generated by the tissue damaged. Uncontrolled production of free radicals
accelerates the oxidative stress and increased the bone remodeling process ultimately
causes osteoporosis. One of the most damaging effects of free radicals is lipid peroxidation;
end product of which is MDA, it also act as major factor in osteoblastic activity. Low level of
antioxidative defense system found in osteoporotic patients due to the deficiency of vitamin D.
Many important mineral ions removed from bones and risk of bone fragility increases. Current
study is aim to check the antioxidative effect produced from excess reactive oxygen species
compared with low level of vitamin D which is held responsible for higher or lower activity
of bone cells. Study Design: Case Control Study. Setting: Study was conducted at Institute
of Molecular Biology and Biotechnology (IMBB), University of Lahore. Period of Study: One
year. Materials and Methods: Blood samples of 272 post-menopausal osteoporotic women
between the age 49-57 were collected from Jinnah hospital Lahore. While the samples of 92
individuals were served as a control. Concentration of both enzymatic and non-enzymatic
antioxidant such as CAT, GSH, SOD, GPx and GR, vitamin A, C and E and levels of MDA
were estimated spectrophotometrically. While the concentration of IL6, AOPPS, AGEs, TNF-α,
MMP9, Isoprostanes, LDH, cholesterol, triglycerides, free fatty acids and phospholipid were
measured by using commercially available Elisa kits. Results: Blood plasma levels of vitamin
D were significantly lower in osteoporosis patients than in normal subjects. In addition, level
of stress biomarker such as MDA was found to be higher in patients as compared to control.
Due to oxidative stress, level of antioxidants (GSH, CAT, and SOD) was found to be reduced.
Blood cells and many other important minerals are also reduces in patient group from their
normal amount. Conclusion: It concludes that excess production of free radicals over whelms
the antioxidative system, thus it may leads to osteoporosis. Further more antioxidant species
subjected to body might protect bone loss and also help in acceleration of healing of fractured