INSINUATIONS OF UPSTREAM PROPHETIC VARIABLES AND THEIR INTERPLAY TO DEVELOP ENDOMETRIOSIS IN YOUNG FEMALES
Keywords:Melondialdehyde (MDA), Vitamin-D, Matrix Metalloproteinases (MMPs), Interleukins (ILs), Testosterone
Objectives: To evaluate the role of endometriosis leading to infertility in working
females. Data Source: Data was collected and screened from Jinnah Hospital Lahore. Design
and Study: Comparative cross sectional study was performed. Setting: Study was carried
out in the Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore.
Period: Present study was carried out over a period of two years from 14-11-2014 to 15-11-2016.
Material and Methods: Hundred (n=100) patients of endometriosis and hundred (n=100)
controls were added in the current study. Subjects were analyzed for the levels of MDA, SOD,
CAT, GSH, ILs, MMPs, Vit-D, PGE-2, Estradiol, COX-2 and various hormones with the help of
their respective spectrophotometric and kit protocols. Results: Levels of MDA, IL-8, MMP-2,
TNF-á and Estradiol were significantly higherin patients of endometriosis (2.99±0.16 nmol/ml,
19.65±3.26 pg/ml, 563.23±48.2 ng/ml, 31.29±4.25 pg/ml and 47.16±4.28 pg/ml) vs. healthy
females (0.88±0.089 nmol/ml, 10.26±1.99pg/ml, 299.35±54.4 ng/ml, 21.26±3.26pg/ml and
22.28±4.16pg/ml), whereas levels of SOD, CAT and GSH remained decreased significantly in
patients infected by endometriosis (652.23±42.6, 1226.06±108.4 and 6.28±0.42 respectively).
Levels of testosterone and 17â-HSD-II were also significantly decreased females with
endometriosis (15.23±1.99 ng/dL and 0.12±0.04 pg/ml respectively). Conclusion: The current
study indicates role of oxidative stress and vitamin-D in females with endometriosis. Lower
levels of vitamin-D and increased oxidative stress is responsible for release of induced factors
i.e., PGE-2 and COX-2. These factors play their role in activation of signaling cascades involved
in progression of endometriosis. Hence, vitamin-D and antioxidant supplementation may have
a beneficial role in the disease management.