Association of P53 expression with clinicopathological gradings of oral squamous cell carcinoma patients in Hyderabad Sindh.

Authors

  • Waqas Iqbal Isra Dental College, Isra University, Hyderabad.
  • Uzma Tariq Isra Dental College, Isra University, Hyderabad.
  • Shahzaman Memon Bhitai Dental and Medical College, Mirpurkhas.
  • Khushbu Lohana Ziauddin College of Dentistry, Ziauddin University.
  • Hafiz Mahmood Muhammad Medical and Dental College, Mirpurkhas.
  • Ayesha Iqbal Indus Hospital, Karachi.

DOI:

https://doi.org/10.29309/TPMJ/2023.30.05.7401

Keywords:

Histological Grading, Immunohistochemistry, P53, Squamous Cell Carcinoma

Abstract

Objective: To evaluate the frequency and association of p53 gene with increase histopathology categorizing of “oral squamous cell carcinoma” patients. Study Design: Comparison Cross-sectional Research. Setting: Isra Dental College Hyderabad. Period: 1 November 2021 to 31 October 2022. Material & Methods: Sixty patients of identified oral squamous cell carcinomas were chosen by consecutive sampling from patients reporting at Oral surgery department Isra dental college, Poly-L-lysine solutions was used over all of the formalin-fixed, paraffin embedded segments, and was prepared for immunostaining. Primary antibody and counter stain hematoxylin was used and dehydrated through graded alcohol dilutions. Sections were mount by Dpx and results interpreted. Results: Sixty instances of oral squamous cells carcinoma divided into histological grading well differentiated OSCC, moderately differentiated OSCC, and poor differentiated OSCC based on their histological characteristics. Fifty-two (87%) of the participants in this study were male, while just eight (13%) were females. Association of p53 were found insignificant with age. Gender and other clinical factors of OSCC, the prevalence of p53 gene countenance was found increase in oral squamous cell carcinoma. But there was no any significant association found with the increase histological grading of OSCC. Conclusion: Prevalence of P53 gene expression found increase in OSCC. While P53 expression was not significantly found with increase histological grading of oral squamous cell carcinoma.

Author Biographies

Waqas Iqbal, Isra Dental College, Isra University, Hyderabad.

BDS, MDS (Oral Pathology), Associate Professor Oral Pathology, 

Uzma Tariq, Isra Dental College, Isra University, Hyderabad.

BDS, MDS (Oral Pathology), Associate Professor Oral Pathology, 

 

Shahzaman Memon, Bhitai Dental and Medical College, Mirpurkhas.

 

BDS, MSc (Oral Pathology), Assistant Professor Oral Pathology, 

Khushbu Lohana, Ziauddin College of Dentistry, Ziauddin University.

BDS, MSc (Oral Biology), Senior Lecturer Oral Biology, 

 

Hafiz Mahmood, Muhammad Medical and Dental College, Mirpurkhas.

 

BDS, M.Phil (Science of Dental Materials), Associate Professor Dental Materials, 

Ayesha Iqbal, Indus Hospital, Karachi.

MBBS, MCPS (Diploma in Diabetes and Endocrinology), Senior Specialist Family Medicine, 

 

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Published

2023-05-02