CITRULLUS COLOCYNTHIS

FRACTIONATION OF METHANOLIC EXTRACT OF CITRULLUS COLOCYNTHIS FOR SPASMOGENS.

  • Muhammad Saleh Faisal Pak International Medical College, Peshawar.
  • Niaz Ali Institute of Basic Medical Sciences, KMU, Peshawar.
  • Kashif Ali Hospital and Research Centre, Peshawar.
Keywords: Fractionation, Citrullus Colocynthis, Spasmogens

Abstract

Objectives: To evaluate the methanolic extract of Citrullus colocynthis and
its different fractions for possible spasmogenic effects on rat’s ileum model. Study Design:
An experimental study. Setting: Department of Pharmacology, Institute of Basic Medical
Sciences, Khyber Medical University, Hayatabad, Peshawar. Period: August 2016 to March
2017. Methodology: Different fractions i.e n-hexane, chloroform, Ethyl acetate, n-butanol and
aqueous fractions of methanolic extract were tried in concentrations of 0.01, 0.03, 0.1, 0.3, 1.0,
3.0, 5.0, 10 and 15 mg/ml to identify the portion with maximum spasmogens. Results: Aqueous
and n-Butanol fractions showed spasmogenic effects on the spontaneous contractions of rat’s
ileal preparations at the concentrations of 0.1, 0.3, 1.0, 3.0 and 5.0 mg/ml while at 10 and
15 mg/ml, they showed spasmolytic effects. Ethyl acetate and chloroform fractions did not
show significant spasmogenic effects, rather showed spasmolytic effects in rabbit’s jejunal
preparations with respective EC50 values of 0.1283 ± 0.007 mg/ml and 2.703 ± 0.235 mg/
ml. Respective EC50 values for spasmolytic effects of Ethyl acetate and chloroform fractions
upon KCl induced contractions were 5.37 ± 0.515 mg/ml and 12.36 ± 1.484 mg/ml. Respective
EC50 (log [Ca++] M) of ethyl acetate fraction at 1mg/ml vs control were -2.563 ± 0.04 and
-2.846 ± 0.035. Similarly, respective EC50 (Log[Ca++]M) in the absence and presence of
0.1 μM verapamil were -2.45 ± 0.06 and -1.7 ± 0.07. EC50 (log [Ca++] M) for control vs
chloroform fraction at 3 mg/ml and 5 mg/ml were -2.95 ± 0.035 vs -3.02 ± 0.03 and -3.06 ±
0.0264, respectively. Similarly, respective EC50 (Log[Ca++]M) in the absence and presence
of 0.1 μM verapamil were -2.45 ± 0.06 and -1.7 ± 0.07. Conclusion: Thus, it is concluded that
spasmogens concentrated in the residual aqueous fraction followed by n-butanol fraction while
spasmolytic constituents are concentrated in the ethyl acetate fraction followed by chloroform
fraction.

Author Biographies

Muhammad Saleh Faisal, Pak International Medical College, Peshawar.

PhD Scholar, M. Phil, MBBS
Assistant Professor,
Department of Pharmacology,

Niaz Ali, Institute of Basic Medical Sciences, KMU, Peshawar.

PhD, MHPE, B. Pharm
Professor,
Department of Pharmacology,

Kashif Ali, Hospital and Research Centre, Peshawar.

M. Phil, Pharm D
Pharmacist
Shaukat Khanum Memorial Cancer

Published
2018-01-10