Ameliorative effect of Alpha-tocopherol on E-cigarette liquid induced histomorphological changes in adrenal cortex of male Albino rats.

  • Ali Ahmed Islamic International Medical College
  • Mehreen Lashari Bolan University of Medical and Health Sciences, Quetta
  • Muhammad Imran Bajwa Islamic International Medical College
  • Jahanzaib Lashari Bolan University of Medical and Health Sciences, Quetta
  • Syeda Sara Bano Islamic International Medical College
  • Dur Muhammad Bolan University of Medical and Health Sciences, Quetta
Keywords: Alpha-Tocopherol, Adrenal Cortex, E-cigarette Liquid

Abstract

Objective: To study the ameliorative effect of Alpha-Tocopherol (Vitamin E) against E-cigarette induced histomorphalogical changes in adrenal cortex of male Albino rats. Study Design: Randomized Animal Control Trial. Setting: Department of Anatomy, Islamic International Medical College Rawalpindi in collaboration with National Institute of Health (NIH), Islamabad. Period: September 2018 to September 2019. Material & Methods: 30 adult male albino rats were divided in three groups having 10 rats each. Normal diet and distilled water was given to control group (Group I) including 10 rats, for 4 weeks. The Rats of Group II were injected E-cigarette liquid 0.5mg per kg body weight per day diluted in 500ul normal saline intraperitoneally for 4 weeks. The experimental rats in Group III, in addition to E-cigarette liquid also received Vitamin E, 100mg per kg body weight per day mixed in daily diet. Dissection of the animals was done after completion of 4 weeks and adrenal gland was removed for histological analysis. Data was recorded and analyzed in SPSS™ version 21. Results: Alpha-Tocopherol restored the quantitative changes induced by E-Cigarette liquid in adrenal cortex in Group III, which was increased in the rats of Group II who were injected E-Cigarette liquid. Among microscopic parameters Orientation of cells, sinusoidal dilatation was significantly reduced in all adrenal cortex zones by Alpha-Tocopherol in Group III animals. Conclusion: The current study demonstrated that Alpha-Tocopherol had an ameliorative effect on histological changes caused by E-cigarette liquid on adrenal cortex.

Author Biographies

Ali Ahmed, Islamic International Medical College

MBBS, M.Phil

Senior Lecturer Anatomy

Mehreen Lashari, Bolan University of Medical and Health Sciences, Quetta

MBBS, M.Phil

Demonstrator Biochemistry

 

Muhammad Imran Bajwa, Islamic International Medical College

MBBS, M.Phil

Assistant Professor Biochemistry

Jahanzaib Lashari, Bolan University of Medical and Health Sciences, Quetta

MBBS, M.Phil

Associate Professor Physiology

 

Syeda Sara Bano, Islamic International Medical College

MBBS, M.Phil

Senior Lecturer Anatomy

Dur Muhammad, Bolan University of Medical and Health Sciences, Quetta

BDS, M.Phil

Senior Lecture Oral Biology

Published
2021-04-10