Vitamin Cobalamin deficiency in macrocytic anemia reporting at a Tertiary Care Hospital.

  • Kashif Rasheed Shaikh Suleman Roshan Medical College, Tando Adam, Sindh, Pakistan.
  • Shumaila Shaikh Khairpur Medical College, Khairpur Mirs, Sindh, Pakistan.
  • Sadia Tabassum Suleman Roshan Medical College, Tando Adam, Sindh, Pakistan.
  • Shagufta Memon Khairpur Medical College, Khairpur Mirs, Sindh, Pakistan.
  • Umair Ali Soomro Indus Medical College Tando Muhammad Khan, Sindh, Pakistan.
  • Shomail Saeed Siddiqui Indus Medical College Tando Muhammad Khan, Sindh, Pakistan.
  • Haji Khan Khoharo Isra University, Hyderabad, Sindh, Pakistan.
Keywords: Cobalamin Deficiency, Macrocytic Anemia, RBC Indices

Abstract

Objective: Determine the frequency of vitamin cobalamin deficiency in macrocytic anemia cases reporting at tertiary care hospital. Study Design: Cross Sectional study. Setting: Faculty of Medicine and Allied Medical Sciences, Isra University, Hyderabad, Sindh Pakistan. Period: January 2017 to October 2018. Material & Methods: 450 cases of both genders, diagnosed as macrocytic- megaloblastic anemia were studied for the vitamin Cobalamin levels. Cases were collected through non- probability convenient sampling by inclusion and exclusion criteria. Consenting volunteers were asked for blood sampling. 5 mL blood was taken from ante – cubital fossa. Samples were centrifuged and sera were collected for the estimation of vitamin cobalamin by ELISA – assay kit. Continuous and categorical variables were entered in SPSS (version 21.0) and analyzed by Student t-test and Chi-square test respectively at 95% CI (P ≤ 0.05). Results: Male and female comprised 225 (43.3%) and 294 (56.6%) of 519 subjects. Male to female ratio was noted 1.30:1. MCV, MCH and MCHC show statistically significant difference between male and female (P<0.05). MCV in male was 96.8±9.92 fl vs. 105.5±12.04 fl in female (P=0.0001). Normal cobalamin was noted in 15.2% (n= 79) and any type of cobalamin deficiency was noted in 84.7% (n= 440) (P=0.0001). Conclusion: The present study reports frequency of 84.7% Cobalamin deficiency in macrocytic anemia reporting at Indus Medical College Hospital. Further studies are recommended by the treating physicians.

Author Biographies

Kashif Rasheed Shaikh, Suleman Roshan Medical College, Tando Adam, Sindh, Pakistan.

MBBS, M.Phil

Associate Professor Pharmacology

 

Shumaila Shaikh, Khairpur Medical College, Khairpur Mirs, Sindh, Pakistan.

MBBS, M.Phil

Assistant Professor Biochemistry

 

Sadia Tabassum, Suleman Roshan Medical College, Tando Adam, Sindh, Pakistan.

MBBS, M.Phil

Assistant Professor Biochemistry

Shagufta Memon, Khairpur Medical College, Khairpur Mirs, Sindh, Pakistan.

MBBS, M.Phil

Assistant Professor Physiology

 

Umair Ali Soomro, Indus Medical College Tando Muhammad Khan, Sindh, Pakistan.

MBBS, M. Phil

Assistant Professor Hematology

Shomail Saeed Siddiqui, Indus Medical College Tando Muhammad Khan, Sindh, Pakistan.

MBBS, M.Phil

Assistant Professor Pathology

 

Haji Khan Khoharo, Isra University, Hyderabad, Sindh, Pakistan.

MBBS, M.Phil, FCPS

Associate Professor Faculty of Medicine and

Allied Medical Sciences

 

Published
2021-04-10