Frequency of MexA gene in pseudomonas aeruginosa isolated from clinical samples of a Tertiary Care Hospital in Pakistan.

  • Syed Luqman Shuaib Khyber Medical College Peshawar.
  • Amina Gul Khyber Medical College Peshawar.
  • Jawad Ahmed Khyber Medical University Peshawar.
  • Noor Rehman Khyber Teaching Hospital Peshawar.
  • Liaqt Ali Pak International Medical College, Peshawar.
  • Shahina Mumtaz Khyber Medical University Peshawar.
  • Anees Muhammad College of Medical Technoloy Bacha Khan Medical College, Mardan.
Keywords: Frequency, Mex A, Peshawar, Pseudomonas

Abstract

The drug resistance genes are responsible to preserve the Pseudomonas. This also happens in the case of Mex drug efflux pumps and expression increase of MexA, MexB and OprM genes in Pseudomonas aeruginosa, when grown in sub-inhibitory concentrations of antibiotics. Objectives: This study was designed to detect the MexA gene of Pseudomonas aeruginosa resistant strains in tertiary care hospital, Peshawar. Study Design: Cross-sectional study. Setting: Department of Pathology, Khyber Teaching Hospital, Peshawar, Pakistan. Period: 14 months duration from April 2015 to May 2016. Material & Methods: The specimens including burn wound swabs, pus and urine were obtained from different patients and were processed on blood agar and MacConkey medium for isolation and identification. Conventional PCR was performed for the MexA gene on 50 specimens. Results: The simple conventional PCR was done for MexA (The Mex AB OprM operon resistance genes) and the O-antigen acetylase gene (the species-specific gene) separately, gave positive bands for 49 out of the 50 specimens. Our finding confirms the presence of the MexA gene (and hence most probably MexABOprM operon) in 49 out of 50 specimens of Pseudomonas aeruginosa. Conclusion: Among other resistance mechanisms to antibiotics and disinfectants, the MexABOprM efflux pump might have a role.

Author Biographies

Syed Luqman Shuaib, Khyber Medical College Peshawar.

MBBS, M.Phil (Micro)

Assistant Professor Pathology

Amina Gul, Khyber Medical College Peshawar.

MBBS, PhD. (Micro)

Assistant Professor Pathology

Jawad Ahmed, Khyber Medical University Peshawar.

MBBS, M.Phil, PhD. (Micro)

Professor of Basic Medical Sciences

Noor Rehman, Khyber Teaching Hospital Peshawar.

M.Phil (Micro)

Professor Pathology

Liaqt Ali, Pak International Medical College, Peshawar.

MBBS, M.Phil (Micro)

Microbiologist Pathology

Shahina Mumtaz, Khyber Medical University Peshawar.

MBBS, DCP, M.Phil

Professor Pathology

Anees Muhammad, College of Medical Technoloy Bacha Khan Medical College, Mardan.

M.Phil (Micro) MLSc.

Lecturer of MLT.

Published
2020-11-10