Immunostaining status of anaplastic lymphokinase in female breast cancer.

  • Syed Naqeeb Ali Al-Tibri Medical College, Isra University Karachi Campus.
  • Syed Liaquat Ali Al-Tibri Medical College, Isra University Karachi Campus.
  • Ghazala Panhwar Al-Tibri Medical College, Isra University Karachi Campus.
  • Sarwat Batool Al-Tibri Medical College, Isra University Karachi Campus.
  • Anila Qureshi Al-Tibri Medical College, Isra University Karachi Campus.
  • Momina Naseer NICVD Karachi.
Keywords: Anaplastic Lymphokinase, Breast Carcinoma, Immunohistochemical Expression

Abstract

Objectives: To determine the status of ALK immunostaining in female breast cancer and to find out the relationship of ALK expression with clinicopathological variables. Study Design: Cross Sectional study. Setting: Department of Pathology Al Tibri Medical College and Hospital Karachi and BMSI Jinnah Post Graduate Medical Centre, Karachi. Period: October 2016 to March 2017. Material & Methods: Total of 110 female cases with breast carcinoma by using non-probability purposive sampling technique. After taking informed consent from the patients, tissue samples were taken from received specimen of mastectomy for hematoxylin and eosin stain. The immunohistochemistry for ALK was assessed by using a DAKO monoclonal antibody using the paraffin embedded blocks of the diagnosed cases of breast carcinoma. Chi square test was used to find out the significance of differences among the variables. Results: Total of 110 cases with different histological classifications of breast cancer were observed i-e invasive ductal carcinoma 95(86%), invasive lobular carcinoma 9(8%), invasive medullary carcinoma 4(3.6%) and papillary carcinoma 2(1.8%). ALK expression was positive in 71(64%) and negative in 39 (35%) patients. Significant association was observed between ALK expression with histological grade (p=0.003), lymph node involvement (p=0.042) and skin involvement (p=0.025). Conclusion: Present study shows higher positive ALK expressions. Present study also indicates significant relationship of ALK with histological grade, lymph node involvement and skin involvement.

Author Biographies

Syed Naqeeb Ali, Al-Tibri Medical College, Isra University Karachi Campus.

MBBS, M.Phil

Assistant Professor Pathology

Syed Liaquat Ali, Al-Tibri Medical College, Isra University Karachi Campus.

MBBS, M.Phil

Senior Lecturer Biochemistry

Ghazala Panhwar, Al-Tibri Medical College, Isra University Karachi Campus.

MBBS, M.Phil

Assistant Professor Biochemistry

Sarwat Batool, Al-Tibri Medical College, Isra University Karachi Campus.

BDS, M.Phil

Senior Lecturer Pathology

Anila Qureshi, Al-Tibri Medical College, Isra University Karachi Campus.

MBBS, M.Phil, Ph.D

Professor Pathology

 

Momina Naseer, NICVD Karachi.

MBBS

Adult Cardiology

 

Published
2020-08-10