Histopathological changes due to arsenic Kushta in testes of wistar rats.

  • Sadaf Shafique Quaid-e-Azam Medical College Bahawalpur.
  • Raheel Khan Bahawal Victoria Hospital, Bahawalpur.
  • A.H. Nagi Chughtai Institute of Pathology, Lahore.
  • Asma Fayyaz Rehmanpura Diagnostic Centre, Rehmanpura, Lahore.
  • Afra Samad Multan Medical and Dental College, Multan.
  • Nudrat Fayyaz Multan Medical and Dental College, Multan.
Keywords: Arsenic, Germinal Epithelium, Histopathological Changes, Interstitium, Kushta, Spermatogenesis

Abstract

Objectives: To see the detrimental effects of arsenic kushta (kushta-e-Sammulfur) induced toxicity in wistar rats. Study Design: Experimental Study. Setting: Department of Morbid Anatomy and Histopathology, University of Health Sciences, Lahore. Period: 02nd June 2016 to 30th September 2016. Material & Methods: This experimental study was conducted on a total of 48 wistar rats, each weighing approximately, 200-300grams. These rats were then randomly divided into four groups each of them comprising of 12 rats. Group I was taken as a control group which was given flour pellets. Group II was given a single dose of 180 mg/kg of arsenic kushta for 2 weeks, whereas Groups III was given 150 mg/kg of arsenic kushta only for 12 weeks and Group IV was also given dose of 150 mg/kg of arsenic kushta along with 75mg of Bovine Serum Albumin for 12 weeks. Histopathological changes were then seen in the germinal epithelium, stages of spermatogenesis and insterstitium of testes of rats. Results: Changes in germinal epithelium, stages of spermatogenesis and interstitium were seen in all the above groups except group 1 which was the control group. Maximum changes were seen in groups C and D which were given high doses of arsenic kushta along with injection of bovine serum albumin. Conclusion: Arsenic kushta preparation of kushta-e-Summulfur causes testicular toxicity in wistar rats and have similar toxic effects in human beings.

Author Biographies

Sadaf Shafique, Quaid-e-Azam Medical College Bahawalpur.

MBBS, M.Phil (Histopathology), FCPS (Chemical Pathology)

Assistant Professor Pathology

Raheel Khan, Bahawal Victoria Hospital, Bahawalpur.

MBBS, FCPS (Medicine)

Senior Registrar Medicine

A.H. Nagi, Chughtai Institute of Pathology, Lahore.

MBBS, Ph.D, FCPS, FCPP, FRCP, FRCPath

Professor Pathology

Asma Fayyaz, Rehmanpura Diagnostic Centre, Rehmanpura, Lahore.

MBBS, M.Phil (Anatomy)

Women Medical Officer

Afra Samad, Multan Medical and Dental College, Multan.

MBBS, M.Phil (Morbid Anatomy & Histopathology)

Associate Professor

Nudrat Fayyaz, Multan Medical and Dental College, Multan.

MBBS, FCPS (Chemical Pathology)

Assistant Professor

Published
2020-08-10