Effect of Eugenia Jambolana on number of inflammatory cells in gingivitis in albino rats.

  • Sana Chaudhry Aviecenna Medical Dental College, Lahore.
  • Behzad Salahuddin Avicenna Medical and Dental College, Lahore.
  • Amna Amanat de’Montmorency College of Dentistry, Lahore.
  • Nadia Bhutta Avicenna Medical and Dental College, Lahore.
  • Muhammad Bilal Arshad Avicenna Medical and Dental College, Lahore.
  • Nasrullah Khan Avicenna Medical and Dental College, Lahore.
Keywords: Buccal Mucosa, Eugenia Jambolana, Gingivitis, Healing

Abstract

Objectives: This study was conducted to study the effect of Eugenia jambolana extract on the thickness of epithelium in albino rats. Study Design: This experimental study. Setting: Conducted in the animal house, Anatomy Department, Post Graduate Medical Institute, Lahore, Pakistan. Period: July to August 2015. Material and Methods: Total 48 albino rats were taken in this study and were divided into three groups. Histological changes of the buccal mucosa were studied on 3rd, 4th, 10th and 20th day after the induction of gingivitis in both control and experimental group. Results: On day 3, the cellular infiltrate was three times in both experimental groups. The total count of neutrophils was 22.50±2.082, eosinophils was 50.00±1.414 in group 1B. In group 1C, the neutrophils count was 27.50±1.915, eosinophil count was 5.00±0.816. On day 4, in group-2B, no eosinophils were seen. Lymphocytes was 19.75±1.708, neutrophils was 6.50±1.219 and macrophages was 3.25±1.258. In group 2C, lymphocytes and macrophages present, very few neutrophils were also seen. Some fibroblast nuclei were seen. In group 2C, no eosinophils were seen. The lymphocytes count was 9.00±1.155, neutrophil count was 4.00±1.414 and macrophage count was 1.25±0.050. On day 10, lymphocytes, plasma cells, macrophages and few neutrophils were seen. In group3B, macrophages with large nucleus 3.25±0.95 lymphocytes 1.25 ±0.500 and few neutrophils 1.00±0.000 were also present. No eosinophils were present. In group 3C, less inflammatory cells (table 4) beneath the epithelium. The subepithelial tissue consisted of more lymphocytes 1.75±0.500 and neutrophils 1.25±0.500.On day 20, very few lymphocytes 1.75±0.500 and neutrophils 1.50±0.577 in experimental group 4B. In group 4C, lymphocytes 1.75±0.500 and neutrophils 1.50±0.577 were present, no evidence of macrophages and eosinophils in group 4B and 4C. Conclusion: This study proved the beneficial effects of Eugenia jambolana on the healing of gingivitis. The contents in Eugenia jambolana have an anti-inflammatory action on soft tissues.

Author Biographies

Sana Chaudhry, Aviecenna Medical Dental College, Lahore.

M.Phil (Oral Biology)

Assistant Professor

Department of Oral Biology

 

Behzad Salahuddin, Avicenna Medical and Dental College, Lahore.

BDS, MSc (Oral Pathology), FICD

Associate Professor

Department of Oral Pathology

 

Amna Amanat, de’Montmorency College of Dentistry, Lahore.

BDS, M.Phil (Oral Biology)

Assistant Professor

Department of Oral Biology

 

Nadia Bhutta, Avicenna Medical and Dental College, Lahore.

BDS, FCPS (Orthodontics)

Professor

Department of Orthodontics

 

Muhammad Bilal Arshad, Avicenna Medical and Dental College, Lahore.

BDS, MSc (DPH)

Assistant Professor

Department of Community and Preventive Dentistry

 

Nasrullah Khan, Avicenna Medical and Dental College, Lahore.

BDS, MCPS (Oral Surgery)

Assistant Professor

Department of Oral and Maxillofacial Surgery

 

Published
2019-11-10