Effect of Eugenia Jambolana on number of inflammatory cells in gingivitis in albino rats.

Abstract

Objectives: This study was conducted to study the effect of Eugenia jambolana extract on the thickness of epithelium in albino rats. Study Design: This experimental study. Setting: Conducted in the animal house, Anatomy Department, Post Graduate Medical Institute, Lahore, Pakistan. Period: July to August 2015. Material and Methods: Total 48 albino rats were taken in this study and were divided into three groups. Histological changes of the buccal mucosa were studied on 3^rd, 4^th, 10^th and 20^th day after the induction of gingivitis in both control and experimental group. Results: On day 3, the cellular infiltrate was three times in both experimental groups. The total count of neutrophils was 22.50±2.082, eosinophils was 50.00±1.414 in group 1B. In group 1C, the neutrophils count was 27.50±1.915, eosinophil count was 5.00±0.816. On day 4, in group-2B, no eosinophils were seen. Lymphocytes was 19.75±1.708, neutrophils was 6.50±1.219 and macrophages was 3.25±1.258. In group 2C, lymphocytes and macrophages present, very few neutrophils were also seen. Some fibroblast nuclei were seen. In group 2C, no eosinophils were seen. The lymphocytes count was 9.00±1.155, neutrophil count was 4.00±1.414 and macrophage count was 1.25±0.050. On day 10, lymphocytes, plasma cells, macrophages and few neutrophils were seen. In group3B, macrophages with large nucleus 3.25±0.95 lymphocytes 1.25 ±0.500 and few neutrophils 1.00±0.000 were also present. No eosinophils were present. In group 3C, less inflammatory cells (table 4) beneath the epithelium. The subepithelial tissue consisted of more lymphocytes 1.75±0.500 and neutrophils 1.25±0.500.On day 20, very few lymphocytes 1.75±0.500 and neutrophils 1.50±0.577 in experimental group 4B. In group 4C, lymphocytes 1.75±0.500 and neutrophils 1.50±0.577 were present, no evidence of macrophages and eosinophils in group 4B and 4C. Conclusion: This study proved the beneficial effects of Eugenia jambolana on the healing of gingivitis. The contents in Eugenia jambolana have an anti-inflammatory action on soft tissues.