Effect of stress induced changes in neuropeptide Y on antioxidant status.

  • Saadia Zainab Al-Nafees Medical College Islamabad.
  • Umar Ali Khan Al-Nafees Medical College Islamabad.
  • Tahir Ahmad Munir Mohi-Ud-Din Islamic Medical College Mirpur AJK.
  • Anjum Ilahi Al-Nafees Medical College Islamabad.
  • Adnan Saleem Khan Al-Nafees Medical College Islamabad.
  • Ayesha Javed Al-Nafees Medical College Islamabad.
Keywords: Malondialdehyde, Neuropeptide Y, Restraint Stress, Superoxide Dismutase

Abstract

In chronic stress, release of catecholamines, adrenocorticoids and pituitary hormones result impaired release of neuromodulator - neuropeptide Y. The deregulated neuropeptide Y results imbalanced redox homeostasis reduced endogenous superoxide dismutase and raised malondialdehyde. Objectives: To find the effect of chronic stress on plasma neuropeptide Y, superoxide dismutase, and malondialdehyde levels. Study Design: Quasi-experimental. Setting: Al-Nafees Medical College & Hospital in collaboration with National Institute of Health, Islamabad. Period: January 2016 to December 2016. Material & Methods: After approval from institutional review board, thirty healthy male Sprague Dawley rats were included in the study and were divided equally into group I (control) and group II (restraint stress). The animals were housed in stainless steel cages, at humidity (40-60%), temperature (22 ± 2°C) and a 12-h light-dark cycle with lights on at 0700 am. After adaptation, group II was exposed to restraint stress of 6 hours daily for 28 days. The blood sampling for plasma neuropeptide Y, serum superoxide dismutase and malondialdehyde levels were taken. Results: There was significant decline in neuropeptide Y plasma and superoxide dismutase serum levels while an increase in malondialdehyde levels serum levels was noticed in restraint stress group. Conclusions: Chronic stress induces decrease in plasma NPY with subsequent increase in serum malondialdehyde and decrease in superoxide dismutase levels.

Author Biographies

Saadia Zainab, Al-Nafees Medical College Islamabad.

MBBS, M.Phil (Physiology)

Assistant Professor Physiology

Umar Ali Khan, Al-Nafees Medical College Islamabad.

MBBS, M.Phil, FCPS, Ph.D (Physiology)

Professor Physiology

 

Tahir Ahmad Munir, Mohi-Ud-Din Islamic Medical College Mirpur AJK.

MBBS, FCPS (Physiology), MRCP (Medicine)

Professor Physiology

 

Anjum Ilahi, Al-Nafees Medical College Islamabad.

MBBS, MRCP (Medicine), FRCP (Medicine),

Dip Card, MRCP (Geriatric)

Associate Professor Medicine

 

Adnan Saleem Khan, Al-Nafees Medical College Islamabad.

MBBS, Dip Card, Master in Public Health

Registrar and Postgraduate Cardiology

 

Ayesha Javed, Al-Nafees Medical College Islamabad.

MBBS, M.Phil (Community Medicine)

Assistant Professor Community Medicine

 

Published
2020-05-10