MYCOBACTERIUM TUBERCULOSIS;

FREQUENCY OF RIFAMPIN RESISTANCE MUTATIONS IN 81-BP RRDR OF RPOB GENE IN MYCOBACTERIUM TUBERCULOSIS ISOLATES IN QUETTA, PAKISTAN.

  • Ghazala Siddique University of Balochistan, Quetta, Pakistan.
  • Muhammad Shafee University of Balochistan, Quetta, Pakistan.
  • Ferhat Abbas University of Balochistan, Quetta, Pakistan
  • Muhammad Naeem University of Balochistan, Quetta, Pakistan.
  • Abdul Raziq University of Balochistan, Quetta, Pakistan.
  • Shafique Ahmad PARC, Quetta.
  • Khan Muhammad Babar Bolan Medical Complex Hospital, Quetta.
  • Ziauddin - University of Balochistan, Quetta, Pakistan.
Keywords: GeneXpert, MDR, Quetta, RIF-resistance, RRDR

Abstract

Objectives: The primary objective of this study is to determine the frequency of rpoB gene mutations within 81-bp RRDR in isolates from Quetta, Pakistan using GeneXpert® MTB/RIF assay. Background: Multi drug resistance is one of the major obstacles in the control of tuberculosis throughout the globe. Study Design: A cross sectional experimental study was designed. Setting: A total of 500 clinical specimens obtained from suspected TB patients at Provincial TB Reference Laboratory, Fatima Jinnah General and Chest Hospital Quetta, Pakistan Period: From January to July 2017. Methodology: Were analyzed by GeneXpert® MTB/RIF assay. Statistical analysis of the data was performed using SPSS version 20. Results: Out of total 500 samples, MTB was detected in 211 (42%) cases by GeneXpert® MTB/RIF assay [positive pulmonary cases 48.8% (206/422) and extra-pulmonary 6.4% (5/78)]. Among 211 MTB positive cases, the assay detected 11 (5.2%) cases with RIF-resistance caused by various rpoB gene mutations within 81-bp RRDR. All the eleven RIF-resistant isolates were found to have mutations only in Probe E and none of cases had RIF-resistance associated with probes A, B, C, and D. Out of 11 RRD cases, 4 (2%) were males and 7 (3.3%) were females. New TB cases were 3 (1.4%) and previously treated cases were 8 (3.8%). Conclusion: In our settings 11/211 (5.2%) of the TB patients showed rifampicin resistance. Probe E mutations (also called codons 531 and 533) were the only rpoB gene mutations detected by GeneXpert® MTB/RIF assay. No mutations were detected in the codons 511, 513, 516, 518, 522 and 526 sequences.

Author Biographies

Ghazala Siddique, University of Balochistan, Quetta, Pakistan.

M.Phil. Scholar

Center for Advanced Studies in Vaccinology & Biotechnology,

 

Muhammad Shafee, University of Balochistan, Quetta, Pakistan.

PhD.

Assistant Professor

Center for Advanced Studies in Vaccinology & Biotechnology,

 

Ferhat Abbas, University of Balochistan, Quetta, Pakistan

PhD.

Professor

Center for Advanced Studies in Vaccinology & Biotechnology,

 

Muhammad Naeem, University of Balochistan, Quetta, Pakistan.

M.Phil.

Center for Advanced Studies in Vaccinology & Biotechnology,

 

Abdul Raziq, University of Balochistan, Quetta, Pakistan.

M.Phil.

Department of Statistics,

 

Shafique Ahmad, PARC, Quetta.

M.Phil.

Balochistan Agriculture & Development Center,

 

Khan Muhammad Babar, Bolan Medical Complex Hospital, Quetta.

MBBS, FCPS

 

Ziauddin -, University of Balochistan, Quetta, Pakistan.

PhD. Scholar

Assistant Professor

Center for Advanced Studies in Vaccinology & Biotechnology,

 

Published
2019-05-10