Immunohistochemical expression of ki-67 in odontogenic keratocyst: Evidence of aggressive behavior.

  • Rabiya Saif de ‘Montmorency College of Dentistry Lahore.
  • Hafiz Majid Jehangir Azhra Naheed dental College, Lahore.
  • Abdul Hannan Nagi University of Health Sciences, Lahore.
  • Nadia Naseem University of Health Sciences, Lahore.
  • Zainab Rizvi de’Montmorency College of Dentistry Lahore.
  • Faiz Rasul de’Montmorency College of Dentistry Lahore.
Keywords: Immunohistochemistry, Ki-67, Odontogenic Keratocysts

Abstract

The odontogenic keratocyst (OKC) well-known for its aggressiveness and high recurrence rate, comprises approximately 11% of all jaw cysts. Due to its aggressive behavior it was placed into category of tumour in 2005 by the World Health Organization (WHO). Objectives: The purpose of this study was to determine the Ki-67 expression in Odontogenic Keratocysts to predict its proliferative potential. Study Design: Descriptive study. Setting: Department of Morbid Anatomy and Histopathology, UHS. Periods: June 2014- June 2018. Material & Methods: This is a descriptive study comprising of 39 cases of odontogenic cysts. These surgically removed samples were processed at University of Health Sciences (UHS) laboratory. Routine staining with Hematoxylin & Eosin stain along with immunohistochemistry (IHC) with Ki-67 antibody was performed. Immunohisto chemical scoring was done on the basis of percentage of the nuclear staining of Ki-67. Data was entered into SPSS 22 and descriptive statistics were measured in the form of percentage and frequency. Quantitative variables such as age of patient, size of the cyst, and Ki-67 score were also measured. P value <0.05 was taken as significant. Results: The mean age of the patients was 25.08 ±14.5 years. Significant association was observed between histological variables with odontogenic keratocyst such as parakeratinized epithelial lining (p = 0.00), epithelial hyperplasia both typical and atypical (p = 0.02) and focal spongiosis (p = 0.04). Foci having epithelial atypia demonstrated stronger staining intensity compared to adjacent normal epithelium. However, no significant association was observed between the histological variables and Ki-67 expression. Conclusion: OKC expressed low Ki-67 expression in most of the cases, however, foci of strong expression were also observed in few cases.

Author Biographies

Rabiya Saif, de ‘Montmorency College of Dentistry Lahore.

BDS, M. Phil Oral Pathology

Demonstrator/ Lecturer

 

Hafiz Majid Jehangir, Azhra Naheed dental College, Lahore.

BDS, M. Phil Oral Pathology

Assistant Professor

 

Abdul Hannan Nagi, University of Health Sciences, Lahore.

MBBS, PhD, FCPS, FCPP, FRCP, FRC PATH

Professor

Department of Pathology

 

Nadia Naseem, University of Health Sciences, Lahore.

MBBS, M. Phil., PhD,

Professor

Department of Pathology

 

Zainab Rizvi, de’Montmorency College of Dentistry Lahore.

BDS, M. Phil Oral Pathology, Ph.D scholar

Associate Professor

 

Faiz Rasul, de’Montmorency College of Dentistry Lahore.

BDS, M. Phil Oral Pathology. M. Phil Public Health

Demonstrator/ Lecturer

 

Published
2020-01-10