POSSIBLE PREVENTION OF REACTIVE OXYGEN SPECIES INDUCED HUMAN TRABECULAR MESHWORK CELL DAMAGE BY RESVERATROL AND ASCORBIC ACID.

  • muhammad yaqoob shahani Liaquat University of Medical & Health Sciences, Jamshoro, Pakistan.
  • umbreen Bano Liaquat University of Medical & Health Sciences, Jamshoro, Pakistan.
  • Shazia Begum Shahani Indus Medical College Tando Muhammad Khan.
  • Pashmina Shaikh Liaquat University of Medical & Health Sciences, Jamshoro, Pakistan.
  • Sameena Gul Memon Liaquat University of Medical & Health Sciences, Jamshoro, Pakistan.
  • Samreen Memon Dow Medical College, DUHS, Karachi, Pakistan.
Keywords: Ascorbate, H2O2, Reactive oxygen, Resveratrol, Trabecular Meshwork

Abstract

Objectives: To analyze the antioxidant activity of Resveratrol and Ascorbic Acid against hydrogen peroxide (an oxidant) mediated cell injury of human trabecular meshwork cells. Study Design: Experimental study. Setting: Molecular Biology Laboratory at Medical Research Centre, Liaquat University of Medical & Health Sciences, Jamshoro. Period: Six months. Materials and Methods: Human Trabecular Meshwork cells were purchased from ScienCell Research Laboratories, USA. TM cell metabolism, TM cell viability and Reactive oxygen species were detected by standard methods in co- and pre- treated TM cells. Results: A significant reduction in TM cell metabolism was observed approximating 61% at 1.0 mM H2O2 compared to Ascorbate – 99% and Resveratrol 99% (p=0.0001). Resveratrol was more effective than Ascorbate even at 4.0 mM H2O2, the TM cell activity was noted 76%. Compared to H2O2- treated TM cells, resveratrol improved mitochondrial function upto 4.0 mM H2O2 (76%). Compared to co-treatment, the pretreatment shows similar results except at 4.0 mM H2O2. At 4.0 mM H2O2 the pre-treat TM cell metabolic activity was found as 11%, 31% and 47% compared to co-treat as 9%, 31% and 76% in controls, ascorbate and resveratrol groups respectively (p<0.05). Resveratrol shows significant decrease in viability was seen in controls compared to Ascorbate and Resveratrol groups. Cell viability showed statistically significant differences at 2.0 and 4.0 mM H2O2 compared to controls (P=0.0001). For reactive oxygen species (ROS), cells were incubated and with Ascorbate and Resveratrol for 24 hours and TM cells were treated with 0.0mM, 0.5 mM, 1.0 mM, 2.0mM and 4.0mM H2O2. Significant decrease in ROS was noted by Resveratrol compared to Ascorbate. Conclusions: Resveratrol and Ascorbate may prove useful in preventing and delaying the glaucoma, and timely institution of these anti – oxidants may help maintain trabecular meshwork functions and prevent visual loss.

Author Biographies

muhammad yaqoob shahani, Liaquat University of Medical & Health Sciences, Jamshoro, Pakistan.

MBBS; D. Ophth. ; PhD

Lecturer

Department of Anatomy

 

umbreen Bano, Liaquat University of Medical & Health Sciences, Jamshoro, Pakistan.

MBBS; PhD

Lecturer

Department of Anatomy

 

Shazia Begum Shahani, Indus Medical College Tando Muhammad Khan.

MBBS, M. Phil (Anatomy)

Associate Professor

Department of Anatomy

 

Pashmina Shaikh, Liaquat University of Medical & Health Sciences, Jamshoro, Pakistan.

MBBS; M.Phil.

Assistant Professor

Department of Anatomy

 

Sameena Gul Memon, Liaquat University of Medical & Health Sciences, Jamshoro, Pakistan.

MBBS; M.Phil.

Assistant Professor

Department of Anatomy

 

Samreen Memon, Dow Medical College, DUHS, Karachi, Pakistan.

MBBS; PhD

Professor

Department of Anatomy

 

 

Published
2019-05-31