KELOID

PRODUCTION OF KELOID ANIMAL MODEL

Abstract

Keloids and hypertrophic scars are basically an overabundance of fibrosis in cutaneous healing process. Unfortunately, keloids are only found in human beings. So far no successful animal model of keloid has been reported. Objectives: To produce an animal model of keloid. Main Outcome Measure: Animal model of keloid. Design: Prospective quasiexperimental trial. Setting: Department of Pathology and Experimental Research Laboratory, University of Health Sciences, Lahore, Pakistan. Duration: One year. Sample Size: Twelve albino animals i.e. three mice, three rats, three Guinea pigs, and three rabbits. Sampling Technique: Simple Convenience. Material and Methods: Three different techniques of keloid production
were used. First animal of each species was given skin punch excision only; second was given Transforming Growth Factor Beta 1 injection only; and third was given Transforming Growth Factor Beta 1 injection followed by skin punch excision. Results were noted at four weeks. Results: Out of twelve animals, keloid was produced only in one rabbit (ear) which was given Transforming Growth Factor Beta 1 injection followed by skin punch excision. Conclusion: Keloid animal model can be produced in rabbit ear by giving Transforming Growth Factor Beta 1 injection followed by skin punch excision of ventral surface skin.