APRICOXIB

EVALUATION OF PRECLINICAL CYTOTOXIC EFFECTS OF APRICOXIB ON BREAST CANCEROUS CELL LINES: AN IN VITRO TRIAL

Authors

  • Fatima Rizvi Dow International Medical College (DUHS), Karachi.
  • Syed Mahboob Alam BMSI, JPMC.
  • Farah Asad JSMU, Karachi.
  • Hina Shams Dow International Medical College (DUHS), Karachi.

DOI:

https://doi.org/10.29309/TPMJ/18.4781

Keywords:

MTT assay, IC50, SI, MCF-7, MCF-10, MDA-MB-231, Apricoxib

Abstract

Background: Breast cancer is most frequently diagnosed cancer globally but there is not any ideal economical and safer agent that not only decreases the progression but also resolve complexities associated with breast cancer such as inflammatory conditions. There was strong link between inflammation and cancer specially breast cancer. Thus by inhibiting the COX enzyme may inhibit the progression of cancer beside of its role in inflammatory conditions of breast. Study Design: Interventional In Vitro trial. Setting: Department of Pharmacology in alliance with PCMD. Period: The duration of study from April 2016 to February 2017. Methodology: For this purpose we used five cancerous lines MCF-7, MDA-MB-231, MCF-10, HT-29 and Hela cell lines. For demonstrating the cytotoxic effects of Apricoxib we used MTT assay (for all cell Lines) and Trypan blue dye exclusion assay (Primarily for MCF-7 cell lines). For calculation of minimum dose required for exert cytotoxic effects of Apricoxib and its selectivity
towards cancerous cells of breast tissue we calculated its IC50 value and Selectivity Index (SI) by MTT assay. Results: Apricoxib significantly reduce the viability of MCF-7, MDA-MB-231, Hela, HT-29 as assessed by MTT assay in dose dependent manner (χ2 (2) = 26.483, p<0.001), (χ2 (2) = 26.49, p<0.001), (χ2 (2) = 26.062, p<0.001) and (χ2 (2) = 26.062, p<0.001) respectively. However Apricoxib had non-significant effects on % viability of MCF-10 cell line (χ2 (2) = 4.167, p=0.654) as assessed by MTT assay. Furthermore Apricoxib had lowest IC50 value against MCF-7 cell line. Conclusion: This study demonstrated that beside of primarily anti-inflammatory effects Apricoxib have additional benefits in term of exerting the cytotoxic effects (in vitro) on cancerous cell lines as indicated by reducing the % viability and reducing the Absorbance value of test sample as compare to control. This opens the newer path for researcher to evaluate different aspects of Apricoxib in field of chemotherapy.

Author Biographies

Fatima Rizvi, Dow International Medical College (DUHS), Karachi.

MBBS, M.Phil
Associate Professor
Department of Pharmacology

Syed Mahboob Alam, BMSI, JPMC.

MBBS, M.Phil, Ph.D
Associate Professor
Department of Pharmacology

Farah Asad, JSMU, Karachi.

MBBS, M.Phil
Associate Professor
Department of Pharmacology

Hina Shams, Dow International Medical College (DUHS), Karachi.

MBBS
Lecturer
Department of Pharmacology

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Published

2018-12-08