GENE POLYMORPHISM;

ASSOCIATION OF INTERLEUKIN 6 RECEPTOR GENE POLYMORPHISM WITH REDUCED BONE DENSITY IN POSTMENOPAUSAL WOMEN.

  • Shoaib Ahmed Rai Medical College, Sargodha
  • Bilal Habib Rai Medical College, Sargodha
  • Amna Mubeen Independent Medical College, Faisalabad
  • Imran Aftab Independent Medical College, Faisalabad.
  • Farwa Naqvi Sargodha Medical College, Sargodha.
  • Amal Shoukat University of Sargodha, Sargodha.
Keywords: Single Nucleotide Polymorphism, Reduced Bone Density, Interleukin 6 Receptor, Gene, Deoxyribonucleic Acid, Genetic Predisposition

Abstract

Introduction: Reduced bone density in postmenopausal women is a complex disease with a strong genetic association. Causative factors are both acquired and genetic. Single nucleotide polymorphisms may be associated with genetic predisposition of this condition. Objectives: The purpose of this study is to find out an association between single nucleotide polymorphism (rs4845617) of IL-6R (Interleukin 6 receptor) gene and reduced bone density in cases of postmenopausal women and controls. Study Design: It is a retrospective case-control study. Duration: From March 2017 to Nov 2017. Setting: Different hospitals of Sargodha. Material and Method: About 30 blood samples were collected from postmenopausal women affected with reduced bone density and 30 healthy age-and gender-matched controls. All the blood samples underwent DNA extraction, Polymerase chain reaction and DNA sequencing techniques. Result: In this study, post menopausal women were screened for the rs4845617 in IL-6R gene for their association with reduced bone density. The chi-square test of independence showed that G allele of rs4845617 was significantly associated with reduced bone density in postmenopausal women (OR=2.28, χ2=8.98, p=0.002). Conclusion: Our results signify that this polymorphism may play a role in reduced bone density susceptibility in postmenopausal women.

Author Biographies

Shoaib Ahmed, Rai Medical College, Sargodha

MBBS, M.Phil (Biochemistry)

Assistant Professor, Department of Biochemistry

Bilal Habib, Rai Medical College, Sargodha

MBBS, M.Phil (Physiology)

Assistant Professor, Department of Physiology

Amna Mubeen, Independent Medical College, Faisalabad

MBBS, M.Phil (Anatomy)

Assistant Professor, Department of Physiology

Imran Aftab, Independent Medical College, Faisalabad.

MBBS, M.Phil (Physiology)

Assistant Professor

Department of Physiology

 

Farwa Naqvi, Sargodha Medical College, Sargodha.

MBBS, M.Phil (Pharmacology)

Assistant Professor, Department of Pharmacology

Amal Shoukat, University of Sargodha, Sargodha.

M.Phil (Nutrition)

PhD Scholar Food and Nutrition

Lecturer Department of Food and Nutrition

 

Published
2019-03-10